Echo® liquid handlers
- Accurate and Precise Low Volume Transfer of Antibody and Enzyme Solutions stored in Glycerol using the Echo® Liquid Handler [poster]
- Acoustic, Touchless, Transfer of Protein Crystallography Fluids [poster]
- Aqueous Acoustic Transfer from a 1536-Well Microplate Using the Echo® Liquid Handler [poster]
- 300 Nanoliter qPCR in a One Step Process from Cells to Cp utlizing the Echo® Liquid Handler [poster]
- Labcyte Access™ Laboratory Workstation: A Novel System that Quickly and Easily Integrates the Echo® Liquid Handler into Any Laboratory Workflow [poster]
- Miniaturization of 1536-Well Quantitative PCR Using the Echo Liquid Handler [poster]
- Rapid Liquid Transfer from a 1536-Well Microplate Using the Echo Liquid Handler [poster]
- Accurate and Precise Low Volume Transfer of Glycerol and Biochemical Assay Reagents Using Acoustic Droplet Ejection [poster]
- Keeping DMSO Concentration below 0.05% [applications note]
- Bioluminescence for Drug Metabolism Profiling (Joint with Promega and featured in 'Promega Cell Notes') [applications note]
- How to Qualify the Precision and Accuracy of Your Echo Series 500 Liquid Handler This note describes in a step-by-step process how you can determine the precision of liquid transfer with your Echo 550 or Echo 555 liquid handler. [applications note]
- 297 Distinct Cell Lines: A High-Content Analysis Assay and a Full-Automation design Soley Using Noncontact Liquid Dispensing Journal of the Association for Laboratory Automation, Volume 12, Issue 5, Pages 318-326 R Rodriguez, J Alfonso, C O'Day, Y Ovechkina and C Ward (MDS PharmaServices), JALA 2007; 12(5):318-326 [Article]
- A High-Content Glucocorticoid Receptor Translocation Assay for Compound Mechanism-of-Action Evaluation, M Agler (BMS) et al., Journal of Biomolecular Screening, 2007, Vol. 12, No. 8, 1029-1041 [Article]
- A Model for Improving Efficiency Through Centralization, J Wingfield, et al, (AstraZeneca), American Drug Discovery, April/May 2008 [Article]
- Acoustic droplet ejection in drug discovery, E Heron, R Ellson, J Olechno, Drug Plus International, April/May 2006, pp 22-25 [Article]
- Acoustic Droplet Ejection Technology, J Olechno, R Stearns, R Ellson, E Heron, Innovations in Pharmaceutical Technology, April, 2006, pp 40-43 [Article]
- Automating Compound Management Systems, Catherine Shaffer, Drug Discovery & Development, August 2005, pp 36-41 [Article]
- Cellular Inhibition of Checkpoint Kinase 2 (Chk2) and Potentiation of Camptothecins and Radiation by the Novel Chk2 Inhibitor PV1019 [7-Nitro-1H-indole-2-carboxylic acid {4-[1-(guanidinohydrazone)-ethyl]-phenyl}-amide], A. Jobson, et al., J Pharmacol Exp Ther Dec 2009 331:816-826, Epub 2009 Sep 9., ...and then 5 nL of compound was delivered into Chk2 and substrate mixture by acoustic technology using Echo 550 (Labcyte Inc. ...) [Article]
- Debunking the Myth: Validation of Fluorescein for Testing the Precision of Nanoliter Dispensing, D L Harris and M Mutz, JALA, 11(4), 233-239, 2006 [Article]
- Improved Screening for Better Lead Generation Acoustic Dispensing at Forefront of New Techniques, L Dunlap, GEN News, Vol 27, No. 20, Nov 15, 2007 [Article]
- Improving IC50 Results with Acoustic Droplet Ejection, J Olechno, J Shieh, R Ellson, JALA, 11(4), 240-246, 2006 [Article]
- Miniaturization and Low Volume Liquid Handling Take the Lead in Lab Automation, B Tulsi, Bioscience Technology [Article]
- Optimizing the Profiling of Protein Kinases Genetic Engineering and Biotechnology News, May 15, 2008, Vol 28, No 10 [Article]
- Precision Nanoliter Aqueous Transfer, J Shieh, S Pickett, J Olechno, GEN News, Vol 27 No 9, May 1 2007 [Article]
- Serial vs Direct Dilution Time to apply new thinking to IC50 determination and dose response analysis? J Comley (HTStec Inc), Drug Discovery World, Spring 2007, 36-50 [Article]
- Small-molecule screen identifies inhibitors of the neuronal K-C1 cotransporter KCC2, E. Delpire et al., Vanderbilt University Medical Center, Proc Natl Acad Sci, Mar 31, 2009, 106(13): 5383-5388 [Article]
- The Effects of Small Molecule and Protein Solutes on Acoustic Drop Ejection, J Shieh, R N Ellson and J Olechno, JALA, 11(4), 227-232, 2006 [Article]
- Tools and Technologies that Facilitate High Throughput Screening, by J Comley, Chapter 3 in High Throughput Screening in Drug Discovery (Methold and Principals in Medicinal Chemistry), ed J Huser, Wiley-VCH, 2006 [Article]
- Echo 550 (Click Here to Open) [Movie]
- Movie of acoustic droplet ejection into a well filled with aqueous buffer. This movie shows that when the dense solution of DMSO is ejected into an inverted solution of buffer, the DMSO stays at the meniscus until the plate is returned to the normal position. The DMSO solution then diffuses gradually rather than as a bolus. [Movie]
- Stroboscopic movie of 2.5 nL droplets being ejected from a single well. This movie captures many individual droplets into one, easy to understand sequence. [Movie]
- Acoustic Droplet Ejection and Laser Scanning Platforms Enable High-Throughput Cell Dispensing and Quantitation S. Miller, J. Shieh, S. Pickett, Labcyte and Blueshift Technologies [poster]
- Acoustic Droplet Ejection Improves Dose-Response Determinations [poster]
- Best Practices for Assessing Nanoliter Transfer Performance for Labcyte Echo Liquid Handlers Using the Artel MVS [poster]
- Compound Transfer to 3456-Well Assay Plates Using the Echo™ 550, F. Masse, S. Guiral, S. Kargman and C. Brideau, Merck Frosst Centre for Therapeutic Research [poster]
- Debunking the Myth - Using Fluorescein in Analytical Measurements of Fluid Transfers [poster]
- Elimination of Serial Dilution [poster]
- Elimination of Serial Dilutions -Improving Throughput in High-Density Format Microplates While Eliminating Cost and Improving Results [poster]
- High Content Screening with sub-wavelength optical imaging of cells transfered acoustically into sub-microliter wells A. Cabasug, M. Mutz, R. Stearns [poster]
- High Throughput Secondary Screening - 1400 Dose-Response Curves Per Hour [poster]
- Impact of Compound Concentration in Solutions on Acoustic Detection and Transfers [poster]
- Improving IC50 Analyses by Reducing Compound Waste, Compound Precipitation, Accumulated Error and Consumables Cost [poster]
- Integrating Acoustic Droplet Ejection into the Laboratory Environment [poster]
- Keeping DMSO Concentration Below 0.5% to Minimize its Effect in HTS Assays [poster]
- Kinase Assay Development using Acoustic Droplet Ejection Technology [poster]
- Label-Free Characterization of Proteins Using Acoustic Droplet Ejection Deposition and Advanced X-Ray Technology Measurement, E. Birnbaum, S. Baldwin, L.J. Peterson-Court, B. Warner (Caldera Pharmaceuticals), S. Picket, J. Shieh (Labcyte) [poster]
- Low Nanoliter Acoustic Transfer of Aqueous Fluids with High Precision and Accuracy [poster]
- Luminescent Assay Optimization, Kinase Profiling, and µHTS Application [poster]
- Miniaturized GPCR Signaling Studies in 1536-well Format [poster]
- Miniaturized Luminescent Metabolism Profiling Assays, T. Worzella, B. Larson, J. Shieh and A. Gallagher [poster]
- Multiple Arraying Applications are Enhanced by Touchless Acoustic Droplet Ejection [poster]
- Nanoliter Transfers in 1536-Well Format Using Acoustic Droplet Ejection [poster]
- New Tool for Automating Serial Dilutions for Activity ConfirmationExperiments, J. Cesarek and D. Nie, AstraZeneca [poster]
- Optimum Conditions for Thermal Sealed Plates with Acoustic Liquid Transfers J. Shieh, M. Gomez, J. Barco, I. Yates. [poster]
- Pharmacological Evaluation of Different Compound Dilution and Transfer Paradigms on an Enzyme Assay in Low Volume 384-well Format, T. Spicer, Y. Fitzgerald, N. Burford, S. Matson, M. Chatterjee, M. Gilchrist, J. Myslik and J. O'Connell, Bristol-Myers Squibb [poster]
- Spot-On-Spot Ultra-Low-Volume Array Applications Using Acoustic Droplet Ejection [poster]
- Testing the Precision of Acoustic Droplet Ejection - Measuring CVs for 5 Nanoliter Transfers [poster]
- The Microliter Assay Limbo [poster]
- Use of Acoustic Droplet Ejection to Transfer human Transfected Cells, S. Gul, H. Poyntz, D. Powell, GSK [poster]
- Using Acoustic Droplet Ejection for Aqueous Samples - Transfer of Protein Solutions [poster]
- Utilisation of the Labcyte Echo® Dose-Response Software for Compound Profiling in Lead Generation, D. Hornigold, D. Swift, D. Laughton, AstraZeneca [poster]
- Validation of Low Volume 1536 "Assay Ready" Compound Plates for a Nuclear Hormone Receptor Screen, H. Eriksson, J. Brengdahl, A. Hogner, B. Becker, AstraZeneca [poster]
- Acoustic Non-Contact Dispensing-The Right Choice for UHTS, presentation by T Spicer of Bristol-Myers Squibb, 10th Anniversary Meeting of the Society for Biomolecular Screening, September 2004 [Presentation]
- DMSO Compound Library Volume Measurement and Hydration Monitoring in Storage Tubes and Microplates, presentation, LabFusion 2004 [Presentation]
- The Echo 550 Delivering Results in Screening, J Zewinski, Bristol-Myers Squibb presentation at LabAutomation 2006 [Presentation]
- Echo® 555/550/520 Liquid Handlers [Specification sheet]
- Echo® Qualified 1536-Well Low Dead Volume Source Microplate (1536LDV) [Specification sheet]
- Echo® Qualified 1536-Well High Base Source Microplate (1536HB) [Specification sheet]
- Echo® Qualified 384-Well Low Dead Volume Source Microplate (384LDV) [Specification sheet]
- Echo® Qualified 384-Well Polypropylene Source Microplate (384PP) [Specification sheet]
- Determining Fill-Volume Working Ranges for Echo Qualified Plates in Your Laboratory [User Note]
- Optimizing Heat Sealing of Labcyte Echo Qualified COC Source Plates with the Velocity11 PlateLoc® [User Note]
- Working Range Volume Calculator [User Note]
- Acoustic Droplet Ejection – New Liquid Handling Technology, New Challenges in Automation and Implementation into the Work Flow Lynn Rasmussen, Clinton Maddox, Sara McKellip, and E. Lucile White of the Southern Research Institute [poster] (see also http://www.southernresearch.org/contract-services/high-throughput-screening.html)
- Use of Aequorin for G protein-Coupled Receptor Hit Identification and Compound Profiling, S. Brough, P. Shah (GlaxoSmithKline), Methods in Molecular Biology, Vol 552, pp 181-193, Aug 2009, Low-volume liquid handlers: Echo550 liquid handler (Labcyte, Sunnyvale, CA) for dispensing low nanoliter reagent volumes into 1536-well plates [Article]
Portrait® spotter
- Acoustic Droplet Ejection Enables Precise Timing and Positioning for Deposition of Matrix to Optimize MALDI Tissue Imaging, B. Browning, S. Hinkson, S. Pickett, P. Chaurand, R. Huang, R. Stearns, R. Ellson, R.M. Caprioli. [poster]
- Multiple Arraying Applications are Enhanced by Touchless Acoustic Droplet Ejection [poster]
- New Acoustic Droplet Ejection Platform Enables Spot-on-Spot Picoliter Reagent Deposition for MALDI Imaging Mass Spectrometry, Arraying and Other Applications [poster]
- Spot-On-Spot Ultra-Low-Volume Array Applications Using Acoustic Droplet Ejection [poster]
- Use of Acoustic Droplet Ejection to Prepare Biochemical Reactions on Tissue Sections for MALDI Imaging Mass Spectrometry, S. Pickett, M. Reid Groseclose, J. Shieh, L. Manier, M. Reyzer, P. Chaurand, R.M. Caprioli, [poster]
- Proteomic Analysis Illuminates a Novel Structural Definition of the Claustrum and Insula, B. Mathur, R. Caprioli, A. Deutch (Vanderbilt University Medical Center), Cerebral Cortex 2009 19(10):2372-2379, [article]
- Portrait® 630
Deerac™ LX reagent dispenser
Deerac™ GX/HTS reagent dispenser
- Deerac GX Series Brochure
- A Bioluminescent-Based, HTS-Compatible Assay to Monitor G-Protein-Coupled Receptor Modulation of Cellular Cyclic AMP M Kumar (Promega), K Hsiao (Promega), J Vidugiriene (Promega), SA Goueli (U Wisc Med School) ASSAY and Drug Development Technologies 5(2): 537-246 (2007) [Product Note]
- A Single Nucleotide Polymorphism on Exon-4 of the Gene Encoding PPAR Is Associated with Reduced Height in Adults and Children, L. Burch et al., The Journal of Clinical Endocrinology & Metabolism Vol. 94, No. 7 2587-2593, Reaction Mixture were dispensed to 384 wells using a Deerac high-speed nanodispenser [Article]
- A-100 Low Volume Pipetting in SNP Genotyping [applications note]
- A-110 Low Volume dispensing for DNA Sequencing at the Broad Institute [applications note]
- A-140 Spotted Arrays [applications note]
- A-150 Automated Colony Spotting [applications note]
- A-160 Assay optimization and profiling in uHTS format [applications note]
- A-180 Automated Dispensation of Yttrium Oxide SPA Imaging Beads [applications note]
- A-190 Miniaturising Real Time PCR Reactions [applications note]
- A-200 Wash Procedures to Minimise cDNA [applications note]
- A-210 DNA Sequencing at the Gallo Research Centre [applications note]
- A-300 HTS Cell-Based Assay (Joint with Promega and featured in 'Promega Cell Notes') [applications note]
- A-310 HTS Kinase Screening (Joint with Promega and featured in 'Promega Cell Notes') [applications note]
- A-320 Miniaturizing P450-GLO Assays (Joint with Promega and featured in 'Promega Cell Notes') [applications note]
- A-330 Performing Multiplexed Cell-Based Assays on an Automated Platform (Joint with Promega and featured in 'Promega Cell Notes') [applications note]
- A-340 Optimizing Kinase Assays for Ultra high-Throughput Profiling (Joint with Promega and featured in 'Promega Cell Notes') [applications note]
- A-350 Bioluminescence for Drug Metabolism Profiling (Joint with Promega and featured in 'Promega Cell Notes') [applications note]
- High Speed FRET based SNP Genotyping Measurement on the PHERAstar [applications note]
- Miniaturizing of Real-Time PCR Reactions This note describes how the Deerac HTS system was used to reduce the volumes of rt-PCR while maintaining high quality results [applications note]
- Optimizing Kinase Assays for Ultrahigh-Throughput Profiling Using the Kinase-Glo Plus Assay [applications note]
- 1536 Application for HitHunter cAMP XS + using Deerac Equator HTS [applications note]
- Deerac GX Gradient Dispense [Movie]
- Stacker Integration [Movie]
- Deerac GX Plate Filling [Movie]
- Assay Development and Miniaturization of the ADP Hunter HS Assay [poster]
- Assay Optimization and Profiling in uHTS Format. [poster]
- Automated Dispensation of Yttrium Oxide SPA Imaging Beads, into 1536-Well Plates using Deerac Fluidics' Equator HTS Pipetting System [poster]
- Automated Miniaturization of Promega's Kinase-Glo® Luminescent Kinase Assay in low-Volume 384 & 1536-Well Formats [poster]
- Automated Multiplexed Cell Based Assays for High Throughput Discovery [poster]
- [POSTER] Automating Promega's CellTiter-Glo ® Luminescent Cell Viability and Caspase-Glo® 3/7 Assays in Low Volume 384 & 1536-Well Formats [poster]
- Cytochrome P-450 Activity Screening in Low Volume 384 & 1536 Well Formats using Promega's P-450 Glo Assays [poster]
- High Throughput Automation of a Dual Reporter Assay in Low Volume 384 & 1536 Well Plate Formats [poster]
- High Throughput Kinase Selectivity Profiling for Drug Discovery Applications [poster]
- Incorporation of Nanolitre Pipetting Technology into Medium and High Throughput SNP Genotyping Platforms at KBiosciences [poster]
- Keeping DMSO Concentration below 0.5% to Minimise its Effect in HTS Assays [poster]
- Low Volume Dispensing for DNA Sequencing at the Ernest Gallo Clinic and Research Center [poster]
- Luminescent Assay Optimization, Kinase Profiling, and µHTS Application [poster]
- Miniaturization of Liquid Handling Procedures for High Throughput Sequencing at the Broad Institute [poster]
- Miniaturized GPCR Signaling Studies in 1536 Format [poster]
- [POSTER] Miniaturized GPCR Signaling Studies in 1536-well Format [poster]
- Miniaturized Luminescent Metabolism Profiling Assays, T. Worzella, B. Larson, J. Shieh and A. Gallagher [poster]
- Miniaturized Luminescent Metabolism Profiling Assays. [poster]
- Miniaturizing Real-Time PCR Reactions [poster]
- Overcoming the Challenges of Minimizing Dead Volume Requirements in Assay Automation for Lead Optimization [poster]
- P-140 Reagent Reservoir Options for the Equator Series [Product Note]
- P-156 Integration Options for the Equator [Product Note]
- P-190 Equator Plate Cooling Options [Product Note]
MicroClime® lid
- Preserving HTS Assay and Storage Microplates with Localized Environmental Lids, R Ellson, Presentation at Screening Europe 2006, Prague, Czech Republic, February 2006 [Presentation]
- Filling MicroClime® Environmental Lids [applications note] [Applications Note]
- MicroClime® Environmental Lids Reduce Edge Effects in Multi-Well Plates This note describes the MicroClime® lid and explains how it works and some of its applications. [applications note]
- MicroClime® lid filled with DMSO with hand-held-syringe (Click Here to Open) [Movie]
- MicroClime® lid filled with DMSO with Hudson (Click Here to Open) [Movie]
- MicroClime® lid filled with water with FlexDrop (Click Here to Open) [Movie]
- MicroClime® lid filled with water with Hudson (Click Here to Open) [Movie]
- Applying the Labcyte MicroClime® Lid in Biological Assays to Improve Data Quality and Quantity, A. Freeman, M. Fagura, G. Bellfield & J. Unitt, AstraZeneca R&D Charnwood, UK, [poster]
- Compound Preservation in Closed Loop Screening [poster]
- Hydration Free, Automation-Friendly Alternative for Compound Library Storage [poster]
- Putting a Lid on Edge Effects: Protecting Compound Libraries with Lid-Based Environmental Control [poster]
- MicroClime® Environmental Lid [Specification sheet]
- Applying the MicroClime® Environmental Lid in Biological Assays to Improve Data Quality and Quantity [Webinar]
- Automated Compound Handling in Live Cell Screening: An Era of Acoustic Drop Ejection [Webinar]
- High-Throughput Screening with Primary Cells [Webinar]
- Acoustic Droplet Ejection – New Liquid Handling Technology, New Challenges in Automation and Implementation into the Work Flow Lynn Rasmussen, Clinton Maddox, Sara McKellip, and E. Lucile White of the Southern Research Institute [poster] (see also http://www.southernresearch.org/contract-services/high-throughput-screening.html)
Access™ laboratory workstation
POD™ plate assembler