ADME/Tox
Assay Development & Optimization
- A-160 Assay optimization and profiling in uHTS format [applications note]
- A-170 Keeping DMSO Concentration below 0.05% [applications note]
- A-300 HTS Cell-Based Assay (Joint with Promega and featured in 'Promega Cell Notes') [applications note]
- A Bioluminescent-Based, HTS-Compatible Assay to Monitor G-Protein-Coupled Receptor Modulation of Cellular Cyclic AMP M Kumar (Promega), K Hsiao (Promega), J Vidugiriene (Promega), SA Goueli (U Wisc Med School) ASSAY and Drug Development Technologies 5(2): 537-246 (2007) [Article]
- A High-Content Glucorticoid Receptor Translocation Assay for Compound Mechanism-of-Action Evaluation M Agler, M Prack, Y Zhu, J Kolb, K Nowak, R Ryseck, D Shen, ME Cvijic, J Somerville, S Nadler (Bristol-Myers Squibb) and T Chen (St Judes Children's Research Hospital), J Biomol Screen 2007; 12:1029-1041 [Article]
- A Model for Improving Efficiency Through Centralization, J Wingfield, et al, (AstraZeneca), American Drug Discovery, April/May 2008 [Article]
- Optimizing the Profiling of Protein Kinases Genetic Engineering and Biotechnology News, May 15, 2008, Vol 28, No 10 [Article]
- Assay Development and Miniaturization of the ADP Hunter HS Assay [poster]
- Assay Optimization and Profiling in uHTS Format [poster]
- High Throughput Kinase Selectivity Profiling for Drug Discovery Applications [poster]
- Luminescent Assay Optimization, Kinase Profiling and uHTS Application [poster]
- Applying the MicroClime® Environmental Lid in Biological Assays to Improve Data Quality and Quantity [Webinar]
- Automated Compound Handling in Live Cell Screening: An Era of Acoustic Drop Ejection [Webinar]
- Acoustic Droplet Ejection – New Liquid Handling Technology, New Challenges in Automation and Implementation into the Work Flow Lynn Rasmussen, Clinton Maddox, Sara McKellip, and E. Lucile White of the Southern Research Institute [poster] (see also http://www.southernresearch.org/contract-services/high-throughput-screening.html)
- Validation of Low Volume 1536 "Assay Ready" Compound Plates for a Nuclear Hormone Receptor Screen, H. Eriksson, J. Brengdahl, A. Hogner, B. Becker, AstraZeneca [poster]
- Gradient, contact-free, volume transfers minimize compound loss in dose-response experiments [poster]
- Precise and Accurate Reagent Transfers Utilizing Acoustic Droplet Ejection Offers New Opportunities in Assay Setup [ALA 2010 poster]
- High-Precision Acoustic Transfer of Buffered Solutions Containing Surfactant and Protein for Miniaturized Biological Assays [poster]
- Precise and Accurate Transfer of Kinase Enzymes Using Accoustic Droplet Ejection [poster]
Cell-based assays
- A-150 Automated Colony Spotting [applications note]
- A-330 Performing Multiplexed Cell-Based Assays on an Automated Platform (Joint with Promega and featured in 'Promega Cell Notes') [applications note]
- 297 Distinct Cell Lines: A High-Content Analysis Assay and a Full-Automation design Soley Using Noncontact Liquid Dispensing Journal of the Association for Laboratory Automation, Volume 12, Issue 5, Pages 318-326 R Rodriguez, J Alfonso, C O'Day, Y Ovechkina and C Ward (MDS PharmaServices), JALA 2007; 12(5):318-326
- Acoustic Droplet Ejection and Laser Scanning Platforms Enable High-Throughput Cell Dispensing and Quantitation S. Miller, J. Shieh, S. Pickett, Labcyte and Blueshift Technologies [poster]
- Applying the Labcyte MicroClime® Lid in Biological Assays to Improve Data Quality and Quantity, Adrian Freeman, M. Fagura, G. Bellfield & J. Unitt, AstraZeneca R&D Charnwood, UK, [poster]
- Automated Multiplexed Cell Based Assays for High Throughput Discovery [poster]
- Automating Promega's CellTiter-Glo ® Luminescent Cell Viability and Caspase-Glo® 3/7 Assays in Low Volume 384 & 1536-Well Formats [poster]
- High Content Screening with sub-wavelength optical imaging of cells transfered acoustically into sub-microliter wells A. Cabasug, M. Mutz, R. Stearns [poster]
- High Throughput Automation of a Dual Reporter Assay in Low Volume 384 & 1536 Well Plate Formats [poster]
- Miniaturized GPCR Signaling Studies in 1536 Format [poster]
- Multiple Arraying Applications are Enhanced by Touchless Acoustic Droplet Ejection [poster]
- Use of Acoustic Droplet Ejection to Transfer human Transfected Cells, S. Gul, H. Poyntz, D. Powell, GSK [poster]
- Applying the MicroClime® Environmental Lid in Biological Assays to Improve Data Quality and Quantity [Webinar]
- Automated Compound Handling in Live Cell Screening: An Era of Acoustic Drop Ejection [Webinar]
- High-Throughput Screening with Primary Cells [Webinar]
Dose-Response
- A-120 Miniaturization of HTS Assays [applications note]
- A-170 Keeping DMSO Concentration below 0.05% [applications note]
- A Model for Improving Efficiency Through Centralization, J Wingfield, et al, (AstraZeneca), American Drug Discovery, April/May 2008 [Article]
- Acoustic Droplet Ejection Improves Dose-Response Determinations [poster]
- Automated Miniaturization of Promega's Kinase-Glo® Luminescent Kinase Assay in low-Volume 384 & 1536-Well Formats [poster]
- High Throughput Secondary Screening - 1400 Dose-Response Curves Per Hour [poster]
- Improving IC50 Analyses by Reducing Compound Waste, Compound Precipitation, Accumulated Error and Consumables Cost [poster]
- Keeping DMSO Concentration Below 0.5% to Minimize its Effect in HTS Assays [poster]
- Luminescent Assay Optimization, Kinase Profiling, and µHTS Application [poster]
- Miniaturized GPCR Signaling Studies in 1536-well Format [poster]
- Miniaturized Luminescent Metabolism Profiling Assays, T. Worzella, B. Larson, J. Shieh and A. Gallagher [poster]
- Pharmacological Evaluation of Different Compound Dilution and Transfer Paradigms on an Enzyme Assay in Low Volume 384-well Format, T. Spicer, Y. Fitzgerald, N. Burford, S. Matson, M. Chatterjee, M. Gilchrist, J. Myslik and J. O'Connell, Bristol-Myers Squibb [poster]
- Utilisation of the Labcyte Echo® Dose-Response Software for Compound Profiling in Lead Generation, D. Hornigold, D. Swift, D. Laughton, AstraZeneca [poster]
- Preserving HTS Assay and Storage Microplates with Localized Environmental Lids, R Ellson, Presentation at Screening Europe 2006, Prague, Czech Republic, February 2006 [Presentation]
- Automated Compound Handling in Live Cell Screening: An Era of Acoustic Drop Ejection [Webinar]
- High Density IC50 Analyses Using the Echo 550 Liquid Handler [Webinar]
- Validation of Low Volume 1536 "Assay Ready" Compound Plates for a Nuclear Hormone Receptor Screen, H. Eriksson, J. Brengdahl, A. Hogner, B. Becker, AstraZeneca [poster]
High-throughput screening
- High throughput Secondary Screening - 1400 Dose-Response Curves per Hour [poster]
- A-140 Spotted Arrays [applications note]
- A-160 Assay optimization and profiling in uHTS format [applications note]
- A-170 Keeping DMSO Concentration below 0.05% [applications note]
- A-300 HTS Cell-Based Assay (Joint with Promega and featured in 'Promega Cell Notes') [applications note]
- A-310 HTS Kinase Screening (Joint with Promega and featured in 'Promega Cell Notes') [applications note]
- A-320 Miniaturizing P450-GLO Assays (Joint with Promega and featured in 'Promega Cell Notes') [applications note]
- A-340 Optimizing Kinase Assays for Ultra high-Throughput Profiling (Joint with Promega and featured in 'Promega Cell Notes') [applications note]
- Optimizing Kinase Assays for Ultrahigh-Throughput Profiling Using the Kinase-Glo Plus Assay [applications note]
- 1536 Application for HitHunter cAMP XS + using Deerac Equator HTS [applications note]
- 297 Distinct Cell Lines: A High-Content Analysis Assay and a Full-Automation design Soley Using Noncontact Liquid Dispensing Journal of the Association for Laboratory Automation, Volume 12, Issue 5, Pages 318-326 R Rodriguez, J Alfonso, C O'Day, Y Ovechkina and C Ward (MDS PharmaServices), JALA 2007; 12(5):318-326 [Article]
- A Model for Improving Efficiency Through Centralization, J Wingfield, et al, (AstraZeneca), American Drug Discovery, April/May 2008 [Article]
- Improving IC50 Results with Acoustic Droplet Ejection, J Olechno, J Shieh, R Ellson, JALA, 11(4), 240-246, 2006 [Article]
- Miniaturization and Low Volume Liquid Handling Take the Lead in Lab Automation, B Tulsi, Bioscience Technology [Article]
- Optimizing the Profiling of Protein Kinases Genetic Engineering and Biotechnology News, May 15, 2008, Vol 28, No 10 [Article]
- Reporting data from high-throughput screening of small-molecule libraries J Inglese (NHGI, NIH), CE Shamu (Harvard Medical School) and RK Guy (St Judes Children's Research Hospital), Nature Chemical Biology 3, 438-441 (2007) [Article]
- Serial vs Direct Dilution Time to apply new thinking to IC50 determination and dose response analysis? J Comley (HTStec Inc), Drug Discovery World, Spring 2007, 36-50 [Article]
- Small-molecule screen identifies inhibitors of the neuronal K-C1 cotransporter KCC2, E. Delpire et al., Vanderbilt University Medical Center, Proc Natl Acad Sci, Mar 31, 2009, 106(13): 5383-5388 [Article]
- The Effects of Small Molecule and Protein Solutes on Acoustic Drop Ejection, J Shieh, R N Ellson and J Olechno, JALA, 11(4), 227-232, 2006 [Article]
- Tools and Technologies that Facilitate High Throughput Screening, by J Comley, Chapter 3 in High Throughput Screening in Drug Discovery (Methold and Principals in Medicinal Chemistry), ed J Huser, Wiley-VCH, 2006 [Article]
- Compound Transfer to 3456-Well Assay Plates Using the Echo™ 550, F. Masse, S. Guiral, S. Kargman and C. Brideau, Merck Frosst Centre for Therapeutic Research [poster]
- Keeping DMSO Concentration below 0.5% to Minimise its Effect in HTS Assays [poster]
- Kinase Assay Development using Acoustic Droplet Ejection Technology [poster]
- Miniaturized Luminescent Metabolism Profiling Assays. [poster]
- Pharmacological Evaluation of Different Compound Dilution and Transfer Paradigms on an Enzyme Assay in Low Volume 384-well Format, T. Spicer, Y. Fitzgerald, N. Burford, S. Matson, M. Chatterjee, M. Gilchrist, J. Myslik and J. O'Connell, Bristol-Myers Squibb [poster]
- Acoustic Non-Contact Dispensing-The Right Choice for UHTS, presentation by T Spicer of Bristol-Myers Squibb, 10th Anniversary Meeting of the Society for Biomolecular Screening, September 2004 [Presentation]
- Preserving HTS Assay and Storage Microplates with Localized Environmental Lids, R Ellson, Presentation at Screening Europe 2006, Prague, Czech Republic, February 2006 [Presentation]
- The Echo™ 550 Delivering Results in Screening, J Zewinski, Bristol-Myers Squibb presentation at LabAutomation 2006 [Presentation]
- Optimizing Heat Sealing of Labcyte® Echo™ Qualified COC Source Plates with the Velocity11 PlateLoc®[User Note]
- Automated Compound Handling in Live Cell Screening: An Era of Acoustic Drop Ejection [Webinar]
- High-Throughput Screening with Primary Cells [Webinar]
- Acoustic Droplet Ejection – New Liquid Handling Technology, New Challenges in Automation and Implementation into the Work Flow Lynn Rasmussen, Clinton Maddox, Sara McKellip, and E. Lucile White of the Southern Research Institute [poster] (see also http://www.southernresearch.org/contract-services/high-throughput-screening.html)
- Acoustic Transfer of Surfactants and Proteins [poster]
- Optimizing Assay Plate Preparation Automation Using a Flexible High-Throughput Application Architecture [poster]
- Validation of Low Volume 1536 "Assay Ready" Compound Plates for a Nuclear Hormone Receptor Screen, H. Eriksson, J. Brengdahl, A. Hogner, B. Becker, AstraZeneca [poster]
- Miniaturizing RNAi Assays: Acoustic Droplet Ejection Enables Efficient and Reproducible Transfer of siRNA Molecules at Low Concentrations [poster ASHG 2010]
Lead optimization